rabbit anti human nrf2 primary antibody  (Proteintech)

Journal: Clinical and Translational Radiation Oncology

Article Title: Differential regulation of radioadaptation by quercetin between human normal and cancer cells

doi: 10.1016/j.ctro.2025.101099

Figure Lengend Snippet: Quercetin restores NRF2 nuclear translocation in radioadapted MCF10A cells. Representative immunofluorescence images of NRF2 (green) and DAPI (blue) and quantification of the nuclear-to-cytoplasmic NRF2 fluorescence ratio in MCF10A cells 24 h after 5 Gy irradiation with or without prior LDRT and quercetin treatment (Scale bar: 10 μm). Data is shown as mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: Cells were incubated with rabbit anti-human NRF2 primary antibody (1:200; Proteintech, 16396–1-AP), and detection was performed using goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, 11001).

Techniques: Translocation Assay, Immunofluorescence, Fluorescence, Irradiation

Journal: Cancer Cell International

Article Title: Stigmasterol sensitizes endometrial cancer cells to chemotherapy by repressing Nrf2 signal pathway

doi: 10.1186/s12935-020-01470-x

Figure Lengend Snippet: Sequences of primers used for amplification of target genes

Article Snippet: Following antigen retrieval, incubation overnight with rabbit anti-human Nrf2 primary antibodies at 4 °C in a humid chamber, followed by 60-min incubation with biotinylated secondary antibody (Dako, Carpinteria, CA, USA).

Techniques: Amplification, Sequencing

Journal: Cancer Cell International

Article Title: Stigmasterol sensitizes endometrial cancer cells to chemotherapy by repressing Nrf2 signal pathway

doi: 10.1186/s12935-020-01470-x

Figure Lengend Snippet: Nrf2 is overexpressed in endometrial cancer. The expression pattern of Nrf2 in normal endometrium (n = 10), hyperplasia (n = 20) and endometrial cancer (n = 90) was assessed using IHC staining. Representative images were captured at 100× magnification ( a ). The IHC scores in endometrial lesion tissues compared with normal endometrial tissues are presented ( b ). * p < 0.05 compared with normal endometrial tissues. The expression pattern of Nrf2 in endometrial fresh tissue was determined by western blot ( c )

Article Snippet: Following antigen retrieval, incubation overnight with rabbit anti-human Nrf2 primary antibodies at 4 °C in a humid chamber, followed by 60-min incubation with biotinylated secondary antibody (Dako, Carpinteria, CA, USA).

Techniques: Expressing, Immunohistochemistry, Western Blot

Journal: Cancer Cell International

Article Title: Stigmasterol sensitizes endometrial cancer cells to chemotherapy by repressing Nrf2 signal pathway

doi: 10.1186/s12935-020-01470-x

Figure Lengend Snippet: Nrf2 involves in chemotherapy resistance. Nrf2 is high expressed in Nrf2 transfected stable cell line in mRNA level ( a ) and protein level ( b ). CCK8 assay was performed to determine the inhibition effect of cisplatin on cell proliferative activity. *p < 0.05 compared with the control group ( c ). Western blot was used to detect the change of the Nrf2 protein after indicated dose of cisplatin treatment in both parental Ishikawa cells and Ishikawa-Nrf2 cells ( d )

Article Snippet: Following antigen retrieval, incubation overnight with rabbit anti-human Nrf2 primary antibodies at 4 °C in a humid chamber, followed by 60-min incubation with biotinylated secondary antibody (Dako, Carpinteria, CA, USA).

Techniques: Transfection, Stable Transfection, CCK-8 Assay, Inhibition, Activity Assay, Western Blot

Journal: Cancer Cell International

Article Title: Stigmasterol sensitizes endometrial cancer cells to chemotherapy by repressing Nrf2 signal pathway

doi: 10.1186/s12935-020-01470-x

Figure Lengend Snippet: Stigmasterol is the Nrf2 inhibitor candidate. Network pharmacology screens the possible inhibitor candidate. a the molecular structure of stigmasterol. White represents carbon atom, blue is hydrogen atom, red is oxygen atom. The predicted human Nrf2 structural domain 2FLU and 5F72 ( b ) and mouse Nrf2 domain 3WN7 and 4ZY3 ( c ) with which stigmasterol binds. It shows the key amino acid of above structural domain interacting with stigmasterol, including human ( d ) and mouse ( e ) Nrf2 protein

Article Snippet: Following antigen retrieval, incubation overnight with rabbit anti-human Nrf2 primary antibodies at 4 °C in a humid chamber, followed by 60-min incubation with biotinylated secondary antibody (Dako, Carpinteria, CA, USA).

Techniques:

Journal: Cancer Cell International

Article Title: Stigmasterol sensitizes endometrial cancer cells to chemotherapy by repressing Nrf2 signal pathway

doi: 10.1186/s12935-020-01470-x

Figure Lengend Snippet: The predicted results of stigmasterol matches with Nrf2 protein

Article Snippet: Following antigen retrieval, incubation overnight with rabbit anti-human Nrf2 primary antibodies at 4 °C in a humid chamber, followed by 60-min incubation with biotinylated secondary antibody (Dako, Carpinteria, CA, USA).

Techniques:

Journal: Cancer Cell International

Article Title: Stigmasterol sensitizes endometrial cancer cells to chemotherapy by repressing Nrf2 signal pathway

doi: 10.1186/s12935-020-01470-x

Figure Lengend Snippet: Stigmasterol sensitizes endometrial cancer cell to cisplatin by suppression of Nrf2 expression. Western blot was used to detect the effect of stigmasterol on Nrf2 expression ( a ). Stigmasterol enhances the inhibitory effect of cisplatin on EC cell proliferation. Stigmasterol was used at indicated doses; a CCK8 assay ( b , c ) and colony formation assay ( d , e ) were used to assess proliferation. The left panels show representative images of crystal violet-stained colonies. Right panels show comparison of colony numbers following indicated treatments. * p < 0.05 compared with control ( d , e ). The early apoptosis was determined by FCM, *p < 0.05 compared with control ( f , g )

Article Snippet: Following antigen retrieval, incubation overnight with rabbit anti-human Nrf2 primary antibodies at 4 °C in a humid chamber, followed by 60-min incubation with biotinylated secondary antibody (Dako, Carpinteria, CA, USA).

Techniques: Expressing, Western Blot, CCK-8 Assay, Colony Assay, Staining

Journal: Cancer Cell International

Article Title: Stigmasterol sensitizes endometrial cancer cells to chemotherapy by repressing Nrf2 signal pathway

doi: 10.1186/s12935-020-01470-x

Figure Lengend Snippet: Stigmasterol enhances the efficacy of cisplatin through TET1-mediated hydroxymethylation via the Nrf2 signaling pathway. Nrf2-ARE activity assay was performed by luciferase activity determination after stigmasterol treatment with indicate dose. * p < 0.05 compared with control ( a ). Western blot was used to estimate the effect of stigmasterol on Tet1 expression ( b ). Dot-blot assay results of EC cells with stimasterol treatment ( c ). Full length of Tet1 plasmid was tranfected in Ishikawa cells, then stimasterol was used to treat these cells. Western blot was carried out to determine whether tet1 overexpression could block stigmasterol-induced Nrf2 decline ( d ). CCK-8 was used to detect the effect of stigmasterol and cisplatin on the spec-2 cells with low level of Nrf2 due to stablely transfection with Keap1, * p < 0.05, ** p < 0.01 compared with control ( e )

Article Snippet: Following antigen retrieval, incubation overnight with rabbit anti-human Nrf2 primary antibodies at 4 °C in a humid chamber, followed by 60-min incubation with biotinylated secondary antibody (Dako, Carpinteria, CA, USA).

Techniques: Activity Assay, Luciferase, Western Blot, Expressing, Dot Blot, Plasmid Preparation, Over Expression, Blocking Assay, CCK-8 Assay, Transfection

Journal: Oncology Letters

Article Title: Effects of co-treatment with sulforaphane and autophagy modulators on uridine 5′-diphospho-glucuronosyltransferase 1A isoforms and cytochrome P450 3A4 expression in Caco-2 human colon cancer cells

doi: 10.3892/ol.2014.2536

Figure Lengend Snippet: Induction of Nrf2 nuclear translocation in Caco-2 cells was examined by immunocytochemistry. Cells were treated for 24 h with 25 μM SFN, 10 nM rapamycin, or a combination of the two. Images are representative of three independent experiments (magnification, ×630). Nrf2, nuclear factor erythroid 2-related factor 2; SFN, sulforaphane.

Article Snippet: The primary rabbit anti-human polyclonal antibody against microtubule-associated protein 1 light chain 3 (LC3) was purchased from Cell Signaling Technology and the rabbit anti-human polyclonal anti-Nrf2 primary antibody was manufactured by Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Translocation Assay, Immunocytochemistry